SNV-PPILP: refined SNV calling for tumor data using perfect phylogenies and ILP

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SNV-PPILP: refined SNV calling for tumor data using perfect phylogenies and ILP

MOTIVATION Recent studies sequenced tumor samples from the same progenitor at different development stages and showed that by taking into account the phylogeny of this development, single-nucleotide variant (SNV) calling can be improved. Accurate SNV calls can better reveal early-stage tumors, identify mechanisms of cancer progression or help in drug targeting. RESULTS We present SNV-PPILP, a...

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Precision medicine attempts to individualize cancer therapy by matching tumor-specific genetic changes with effective targeted therapies. A crucial first step in this process is the reliable identification of cancer-relevant variants, which is considerably complicated by the impurity and heterogeneity of clinical tumor samples. We compared the impact of admixture of non-cancerous cells and low ...

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Best practices for SNV and methylation calling from bisulfite sequencing data

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MethylExtract: High-Quality methylation maps and SNV calling from whole genome bisulfite sequencing data

Whole genome methylation profiling at a single cytosine resolution is now feasible due to the advent of high-throughput sequencing techniques together with bisulfite treatment of the DNA. To obtain the methylation value of each individual cytosine, the bisulfite-treated sequence reads are first aligned to a reference genome, and then the profiling of the methylation levels is done from the alig...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2014

ISSN: 1460-2059,1367-4803

DOI: 10.1093/bioinformatics/btu755